Stem Cells and Tissue Repair by Unknown

Author:Unknown
Language: eng
Format: epub
ISBN: 9781071606551
Publisher: Springer US

2 Materials

2.1 Material for Dissection

1.Dissection scissors and forceps, dissection needles (insulin needles).

2.Dissection glass dish.

3.Stereoscope.

2.2 Solutions

1.Phosphate-buffered saline (PBS): 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4.

2.Poly-d-lysine: stock 1 mg/ml, dilute to 0.1 mg/ml in distilled, sterile H2O to obtain the working solution.

3.Laminin: stock 100 μg/ml (Sigma-Aldrich, L2020), dilute to 5 μg/ml in neurobasal medium to obtain the working solution.

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